Isolation of Soybean Nodule Bacteria and Nodule Formation in Uzbekistan Soybean Varieties

The main goal of our research work is to search for nodule bacteria local soybean varieties and identify study their nodule-forming properties. In the present study, soil samples from fields in Tashkent, Andijan, Bukhara, Jizzakh, Kashkadarya, Navoi, Namangan, Samarkand, Surkhandarya, Syrdarya, Fergana, Khorezm regions Uzbekistan were studied formation symbiotic nodules varieties. Nodules formed only soils Tashkent region root systems (Madad, Sevinch, Dostlik, Parvoz, Gavkhar, Khasildar, Baraka, Uzbekistan-6, Tumaris, Nafis, Orzu) 22 40 nodule. Forty-one bacterial species belonging genus Bradyrhizobium isolated different specificity, virulence, efficacy 12 active compared Madad, Parvoz these 2 14 pink 0.5 - 10 mm size. Inoculation Madad Dostlik with specific M5-1 D24-1 resulted a efficiency 46.6% 54.4% higher than uninoculated control plants. Notably, foreign inoculum “Rizovit” (Kazakhstan), created on basis japonicum, did not form any roots reason this may be difference genetic origin domestic nucleotide sequences 16S rRNA genes M5-1, S7-2, D24-1, P12-1 showed 97.07% similarity japonicum PRY65 (AF239848.2) 98.98% PRY62 (AF239847.2).